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Workshop on veterinary antimicrobials for microbiologists and pharmacologists, college residents, regulatory authorities, pharmaceutical companies...

VETCAST 2017 workshop

Antimicrobial susceptibility testing with VETCAST breakpoints: quantitative methods

12-15 SEPTEMBRE 2017 TOULOUSE (France) - Hotel Mercure Centre Compans, boulevard Lascrosses, 31000 Toulouse

Conference and Workshop

12 September : overview on VETCAST current standards on AST in veterinary medicine

13-15 September: workshop on the main tools used to determine clinical breakpoints (lectures and practical exercises)

Tuesday 12 September 2017

9:00 am - 5:00 pm : The first-day conference

09:00-9:15 Meet & Greet – An overview of the course (Pierre-Louis Toutain/Dik Mevius)
09:15-10:00 Lecture 1 Current standards for AST in veterinary medicine (Dik Mevius)
10:00-10:30 Lecture 2 Introduction to VETCAST: objectives, current achievements and future plans (Peter Damborg)

11:00-11:45 Lecture 3 CLSI / VAST approaches and experiences (Marilyn Martinez)
11:45-12:30 Lecture 4 Tools for testing: present and future (including innovations in rapid bedside, genetic and other tests) (Alex van Belkum)

Lunch

13:30-14:15 Lecture  5 The setting of clinical breakpoints by VETCAST   (Pierre-Louis Toutain)
14:15-15:00 Lecture  6  Overview on determination of ECOFF, PK/PD breakpoint  and clinical cut-offs (Pierre-Louis Toutain/Alain Bousquet-Melou/John Turnidge)

15:30-16:00 Lecture7 CEESA position and questions (Hilde Moyaert)
16:00-17:00 Open questions and discussion with stakeholders (Peter Lees /Dik Mevius (moderators))

Wednesday 13 September 2017

9:00 am - 5:00 pm : The 3-day workshop

Wednesday 13 September 2017:  ECOFF and introduction to  PK/PD  and clinical cut-offs

9:00-9:30 Lecture 8 MIC wild type distributions and epidemiological cut‐off values: human and veterinary perspectives John Turnidge/Kees Veldman
09:30-10:30 Lecture 9 Methods for defining the wild type distribution (determining the ECOFF) John Turnidge
10:30-11:00 Morning break
11:00-12:15 Exercise 1 ECOFF: practical exercise John Turnidge
12:15-13:45 Lunch
13:45-14:45 Lecture 10 PK/PD indices and experimental in vitro and in vivo approaches: an overview Alain Bousquet-Melou/Aude Ferran
14:45-15:05 Lecture 11 Determination of potency indices in differing matrices Peter Lees
15:05-15:35 Lecture 12 Clinical trials and clinical cut-off for AST: an overview and current issues Pierre-Louis Toutain
15:35-16:05 Lecture 13 Clinical cut-off: the CLSI approach (Maxdif and Window expert system tools) Marilyn Martinez
16:05-16:30 Afternoon Break
16:30-17:00 Lecture 14 Statistics associated with validation of tests and ROC curves Didier Concordet
17:00-18:00 Exercise 2 Computation of specificity and  selectivity of tests; building a ROC curve; computation of positive and negative predictive values and diagnostic gain of  tests Didier Concordet

Thursday 14 September  2017: Population modelling and PK/PD cut-offs

8:45-9:30 Lecture 15 Population modelling: an introduction Pierre-Louis Toutain and Didier Concordet
9:30-10:30 Exercise 3 Step-by-step building of a population model with Phoenix© to estimate typical PK values and random components to establish PK/PD cut-offs and computation of an individual AUC using a Bayesian approach for the computation of a clinical cut-off Pierre-Louis Toutain, Didier Concordet and tutors
10:30-11:00 Morning break
11:00-12:00 Exercise 3 Continued  
12:00-13:30 Lunch
13:30:15:00 Exercise 4 Simulation of dosage regimens using the population model and determination of corresponding PK/PD cut-offs Pierre-Louis Toutain and tutors
15:00-15:30 Afternoon  break
15:30-16:30 Exercise 4 Continued Pierre-Louis Toutain and tutors
16:30-18:00 Exercise 5 Monte Carlo Simulations using Crystal Ball  and computation of dosage regimens with Target Attainment Rates Ludovic Pelligand

Friday 15 September 2017: PK/PD and Clinical cut-offs & clinical  breakpoint

8:30-9:15 Lecture 16 How to analyze classical killing curves to compute critical values of PK/PD indices (Emax model…) Lena Friberg
9:15-10:00 Lecture 17 How to select  PK/PD indices and estimate target values using semi-mechanistic models (demonstration) Lena Friberg
10:00-10:30 Morning break
10:30-11:15 Lecture 18 Classical PK/PD indices re-visited by semi-mechanistic models (  the case of long-acting drugs and formulations)  and future trends  in human medicine Lena Friberg
11:15-12:00 Lecture 19 Classification and regression tree analysis and logistic regression Didier  Concordet
12:00-13:30 Lunch
13:30-14:15 Exercise 6 A logistic regression analysis with Phoenix© Pierre-Louis Toutain/Ludovic Pelligand
14:15-15:00 Exercise 7 A regression tree analysis with R Didier  Concordet
15:00–15:30 Afternoon  break
15:30-16:00 Lecture 20 The VETCAST SOP and Excel sheet  for PK data collection: discussion and update Moderators : Ludovic Pelligand, Peter Lees, Pierre-Louis Toutain
16:00-16:45   End of the workshop and de-briefing Moderators : Pierre-Louis Toutain,  Peter Lees